mouse anti horse iggb  (Bio-Rad)

Journal: Molecular Immunology

Article Title: The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies

doi: 10.1016/j.molimm.2007.06.158

Figure Lengend Snippet: Reactivity of anti-horse IgG antibodies with reqIgGs

Article Snippet: The following antibodies were tested for reactivity against the reqIgGs in ELISA: goat HRP-conjugated polyclonal antibodies specific for horse IgGa, IgGb, IgGc or IgG(T), (kindly provided by Serotec, Oxford, UK and Bethyl Laboratories, Montgomery, TX, USA), and mouse monoclonal antibodies (mAb) against horse IgGa (CVS48), IgGb (CVS39), IgGc (CVS53) and IgG(T) (CVS38) (kindly provided by Serotec).

Techniques:

Journal: Molecular Immunology

Article Title: The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies

doi: 10.1016/j.molimm.2007.06.158

Figure Lengend Snippet: Immunoblot of reqIgGs under reducing conditions untreated or treated with N -glycanase. C, control untreated IgG; T, treated with N -glycanase. IgG1 was probed with anti-horse IgGa; IgG2, IgG3 and IgG5 with anti-horse IgG(T); IgG4 and IgG7 with anti-horse IgGb; and IgG6 with anti-horse IgGc. On each gel, an equivalent quantity of each reqIgG subclass (1 μg) was loaded.

Article Snippet: The following antibodies were tested for reactivity against the reqIgGs in ELISA: goat HRP-conjugated polyclonal antibodies specific for horse IgGa, IgGb, IgGc or IgG(T), (kindly provided by Serotec, Oxford, UK and Bethyl Laboratories, Montgomery, TX, USA), and mouse monoclonal antibodies (mAb) against horse IgGa (CVS48), IgGb (CVS39), IgGc (CVS53) and IgG(T) (CVS38) (kindly provided by Serotec).

Techniques: Western Blot, Control